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KMID : 0352419930120040466
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Keimyung Medical Journal
1993 Volume.12 No. 4 p.466 ~ p.472
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Effect of Parenteral Administration of Endotoxin on Changes of Serum and Hepatic Alcohol Dehydrogenase, Catalase and Aldehyde Dehydrogenase Activities in Rats
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Abstract
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The activities of the alcohol dehydrogenase(ADH), catalase and aldehyde dehydrogenase(ALDH) which are detoxifying enzymes in the liver were measured in order to evaluate the detoxifying ability of the liver to endotoxin.
For administration of endotoxin, a dose of 5mg of endotoxin(lipopolysaccharide E. coli O26: B6 from Sigma chemical company. USA) per kg of body weight was administered through a right external jugular vein. Then the rats were killed after 3, 8
and
24
hours of injection with endotoxin to measure the activities of the above enzymes in serum and their liver.
The activity of the serum ADH showed a statistically significant increase between 3 and 24 hours after endotoxin administration, but that of the cytosolic ADH in the liver showed a significant decrease throughout the experiment. The activity of
catalase
in the liver showed a significant decrease throughout the experiment. The activity of the cytosolic ALDH in the liver showed a significant increase between 3 and 24 hours after endotoxin administration. The activities of the microsomal and
mitochondrial
ALDH in the liver showed a significant decrease between 8 and 24 hours after endotoxin administration respectively.
According to the results, cytosolic ADH and catalase leak into the blood through the damaged membrane of hepatocyte. Microsomal and mitochondrial ALDH leak into the cytosol through their damaged membranes. Ant it is suggested that the synthesis
of
the
microsomal ALDH in the liver is decreased.
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